Research in the department of Molecular Biology headed by prof. dr. H.G. Stunnenberg aims at unraveling the molecular basis of development and differentiation emanating from the genome and epigenome in the context of health and disease. The major focus is on the regulation of gene expression in mammals as well as Plasmodium falciparum, the causative agent of malaria. Novel technological developments that are applied range from single molecule studies through to genome wide elucidation of genetic and epigenetic pathways and mechanisms. An ultra high throughput single molecule sequencing platform is operational in the department to analyze by ChIP-seq for transcription factor binding sites, epigenetic marks, MeDIP-seq and MethylCap-seq for DNA-methylation and RNA-seq at a comprehensive genome wide scale. In depths analysis of the genome wide data is performed and sustained by our own bioinformatic team as well as with outside collaborators. The group participates in a large number of international consortia tackling pathways and molecular mechanisms in embryonic stem cells and pluripotency (HEROIC, PlurySys), nuclear receptor action (TI-Pharma) and cancer (KWF, Epitron, CancerDIP, ICGC). High accuracy mass spectrometric approaches are used to identify and to characterize protein complexes that write, erase and translate epigenetic marks.
The Department of Molecular Biology is embedded in the graduate school Nijmegen Center for Molecular Life Sciences (NCMLS), the main research program Oncology and the Academic Oncology Center (AOCN) of the Radboud University Nijmegen Medical Center.
Persons involved in the project:
Prof. Dr. HG Stunnenberg
Dr. J Martens
Dr. K-J Francoijs